IColohunter
Plasma DNA Methylation Detection Kit for Colorectal Cancer
IColohunter is a blood test for colorectal cancer (CRC) using DNA methylation analysis involves detecting specific methylation patterns in cell-free DNA (cfDNA) in the bloodstream. It detect the DNA methylation of NTMT1 and MAP3K14-AS1 gene in blood.
This approach aims to identify early-stage CRC and potentially precancerous lesions by detecting changes in DNA methylation, an epigenetic modification that can alter gene expression.
IColohunter is a non-invasive screening method to people who are unwilling to be screened by colonoscopy.
The Progression of Colorectal Cancer
The Importance of Early Screening
We know Colorectal Cancer is a high incidence malignant tumor and can be called as CRC and normally we also name it as “lazy cancer”.
It’s not easy to discover and we will possibly miss some warning signals from our body during our every moment.
When people have obvious characteristics of CRC, the so-called five-year survival rate has been less than 12%.
Who's at risk?
Age over 40+
History of colorectal cancer in first-degree relatives
With related symptoms
(such as blood in stool, mucus stool, constipation, diarrhea, thin stool, abdominal pain, etc.)
History of colorectal disease or cancer
（History of any malignancy）
History of precancerous colorectal cancer（Colorectal adenoma, inflammatory bowel disease, colorectal polyps, etc.）
Have a family history of hereditary colon polyps
Unexplained anemia or weight loss
History of chronic appendicitis or appendectomy History of chronic biliary disease or cholecystectomy
The Advantage of IColohunter
Non-invasive
Use blood as sample for detection.
Ultra sensitivity
The sensitivity for CRC is 91.2%, much higher than other non-invasive screen-ing tests (e.g.,FOBT).
Fast result
Results available within 4.5h since sample processing
Simple sampling
Sampling is as simple as a blood draw.
Cost effective
More reasonable price than colonoscopy
Simple testing procedure
Familiar Real-time PCR technology, compatible with mainstream brand instruments
The Comparison between IColohunter with FIT and Colonoscopy
FOBT | FIT | Colonoscopy | IColohunter | |
| Sample | Fecal | Fecal | – | Blood |
| Sensitivity for CRC | 40% | 70% | 75%-95% | 91.42% |
| Specificity for CRC | 98% | 95% | 89%-91% | 91.74% |
| Sensitivity for adenoma | 12% | 22% | 76% | 44.16% |
| Dietary restrictions | Need | No need | Need | No need |
| Invasive | No | No | Yes | No |
| Result Reliability | It can also detect blood from anal fissure or animal blood derived from food. | It can also detect blood from anal fissure or animal blood derived from food. | Gold standard | Reliable |
Kit Components of IColohunter
Plasma cfDNA Extraction and Bisulfite Conversion Kit
Plasma DNA Methylation Detection Kit for Colorectal Cancer (Real-Time PCR)
Testing Procedures
Sample collection
Pretreatment and extraction
Bisulfite conversion and purification
qPCR procedure
Analysis and Report
Sample collection
Pretreatment and extraction
Bisulfite conversion and purification
qPCR procedure
Analysis and Report
Result Interpretation
It is recommended that a positive result should be followed-up with a structural examination of the colon such as diagnostic colonoscopy.
(Note: “Unde” demonstrates no aberrant methylation is detected.）
Gene methylation | Test results of samples | |
| NTMT1 | MAP3K14-AS1 | |
| Positive | Positive | Positive |
| Positive | Negative | |
| Negative | Positive | |
| Negative | Negative | Negative |